Immunohistochemical staining of cholecystokinin (CCK-8) in male genital tract of the rat
in postnatal development (computer analysis of the morphological image) Article published in Urologia Polska 2002/55/4.
authors
-
Bogdan Mi¶kowiak 2, Andrzej Limanowski 1, Małgorzata Partyka 1, Aneta Konwerska
- 1 Katedra i Zakład Histologii i Embriologii Akademii Medycznej w Poznaniu Kierownik katedry: prof, dr hab. Andrzej Łukaszyk
2 Katedra Optometrii i Biologii Układu Wzrokowego Akademii Medycznej w Poznaniu Kierownik katedry: prof, dr hab. Bogdan Mi¶kowiak
keywords
-
male genital tract, testis, epididymis, prostate, seminal vesicles, cholecystokinin (CCK-8), postnatal development, image computer analysis
summary
- The nun of the study. The aim of this study is to establish presence, location and role of cholecystokinin (CCK-8) in the genital tract of
- male rat in postnatal development. Material and methods. Location of cholecystokinin (CCK-8) in testes, epididymal ducts, seminal vesicles and abdominal prostates of the
- rats were determined immunohistochemically from birth to sexual maturity. The intensity of the reaction in secretory cells was
- estimated by computer programme, which showed optical density of the reaction. Results. CCK-8 was observed in a Golgi apparatus region of glandular cells of the prostate and in epididymal ducts in 20 and 35 day,
- respectively. The immunoposilive reaction was seen in seminal vesicles in 35 day and was observed during examination time in all
- cytoplasm of the epithelial cells. In stromal and gamctogenic region of the testis CCK-8-immunopositivc substances were not seen. Conclusions. Our results show the important influence of CCK-8 on a growth and differentiation of epididymal duct, seminal vesicles and
- abdominal prostate.
references
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correspondence
Bogdan Mi¶kowiak
Katedra i Zakład Htsiologii i Embriologii AM
ul. ¦więcickiego 6
60-781 Poznań
tel (0-61) 86-99-181 w. 546
fax (0-61) 86-58-130
e-maih bmiskow@am.poznan.pl
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