Proapoptotic influence of alpha1-antagonists on the homeostasis in prostate epithelium
in patients with benign prostatic hyperplasia. Experiment in vitro Article published in Urologia Polska 2008/61/Supl. 1.
authors
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Łukasz Pokrywka, Zbigniew Wolski, Tomasz Drewa, Bartosz Misterek, Jan Styczyński, Robert Dębski
- Katedra i Klinika Urologii Ogólnej, Onkologicznej i Dziecięcej CM UMK w Bydgoszczy
Katedra i Klinika Pediatrii i Hematologii Dziecięcej CM UMK w Bydgoszczy
Zakład Inżynierii Tkankowej, Katedra Biologii Medycznej CM UMK w Bydgoszczy
summary
Introduction. Stem cells play important role in etiology and development of the prostate diseases. CD133 antigen is one of the main markers for prostatic epithelial stem/progenitor cells. Doxazosin (Dox) induces apoptosis in both the prostatic epithelial and stromal cells.
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Objectives. The aim of study was to compare frequency of the apoptosis induced by doxazosin (Dox) in both stem/progenitor and adult cells of prostate epithelium.
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Materials and methods. Material for cultivate epithelial cells cultures, isolation and cultivate stem/progenitor cells cultures were from 10 BPH patients. After 14 days primary cultures were incubated for 12 hours with 20 (Dox 20), 50 (Dox 50) and 80 μM/l (Dox 80) of doxazosin. Cells viability was estimated with Trypan blue test as well as cells viability and frequency of apoptosis were estimated with Annexin V and propidium iodide test using flow cytometry.
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Results. 90 cultures containing both the stem/progenitor cells (CD133(+)) and adult prostatic epithelial cells (CD133(-)) as well as 41 cultures containing sorted from prostate epithelium stem/progenitor cells (CD133(+)) were established. In CD133(+)/CD133(-) cultures after 12 hours of incubation with Doxazosin significant decrease of living cells percentage and significant increase of apoptotic cells percentage correlating with Doxazosin concetration compared with CD133(+)/CD133(-) cultures incubated without Doxazosin (control) were noted. In CD133(+) cultures there were no significant changes in living and apoptotic cells percentage after 12 hours incubation with Doxazosin comparing with control.
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Conclusions. Doxazosin induces apoptosis in adult prostatic epithelial cells. Proapoptotic influence on stem/progenitor prostatic epithelial cells is insignificant. That phenomenon may have influence on temporary effect of alpha1-antagonists in BPH treatment which is clinically observed.
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