PTU - Polskie Towarzystwo Urologiczne
home page
current numbers
archival numbers
list of articles:
list przewodni/covering letter
editor
Scientific Committee
publisher
subscription
advertising
important links

EVALUATION OF RENAL CELL CARCINOMA TUMORS ANGIOGENIC ACTIVITY IN EXPERIMENTAL CUTANEOUS MODEL IN VIVO
Article published in Urologia Polska 2000/53/1.

authors

Krzysztof Pastewka 1, Ewa Skopińska-Różewska 2, Ewa Sommer 2, Mirosław Kazoń 1, Andrzej Borówka 1
1 Klinika Urologii CMKP w Warszawie
Kierownik: prof. dr hab. med. A. Borówka
2 Zakład Immunologii IGiCHP w Warszawie
Kierownik: prof, dr hab. med. E. Skopińska-Różewska

keywords

kidney RCC carcinogenesis angiogenesis

summary

The aim of the study was to induce angiogenic activity of human renal cell
carcinoma (RCC) tumors in Balb/C angiogenesis test.
Material and method. The difference of angiogenic activity between cancer
cells and phisiological Parker medium and between kidney tumors and normal
kidney tissue homogenates by evaluation of activation ratio (Wp) were
investigated. The experimental test of the in vivo mouse cutaneaus angiogenesis
is valuable method for the assessment of neovascularisation induced by isolated
neoplasmatic cells or tissue homogenates of renal cell carcinoma (RCC).
The assessment of proangiogenic activity in dissection microscope is difficult
as far as differentiation between newly formed blood yessels and background
is concerned (standard of Sidky, Auerbach is obvious).
Results. Injection of 5 x 105 cells per inoculation of RCC caused clear
enhancement of angiogenesis Comparing to control electrolites Parker medium
activation ratio, Wp = 4.47
Comparing to normal renal tissue homogenates (Wp = 1.6).
Conclusions. The authors observed in experimentalmodel in vivo high
angiogenic activity of human RCC tumours.

references

  1. [1] Andrade, S. P., Fan, T. P., Lewis, G. P.: Quantitative in vivo studio on
  2. angiogenesis in a rat sponge model. Br. J. Exp. Pathol. 1987, 68, 755-766.
  3. [2] Auerbach, Sidky: Lymphocyte induced angiogenesis, a quantitative and sensi-
  4. tive assay by modified assay of the graft versus host reaction. J. Exp. Med. 1975,
  5. 141, 1084-1100.
  6. [3] Bałan, B., Skopińska-Różewska, E., Kukiełka, G., Domański, J.: Próba
  7. zastosowania cyfrowych metod analizy i przetwarzania obrazów do oceny proce-
  8. sów angiogenezy indukowanej komórkami ludzkich nowotworów na modelu
  9. zwierzęcym. III Symp. Nauk. Technika Przetwarzania Obrazu. Politechnika
  10. Warszawska, 1997.
  11. [4] Bochner, B. H., Windham, C. Q., Pao, M. M., Kasahara, N., Skinner,
  12. D. G., Jones, P. A.: Overexpression of trombospondin-1 inhibits bladder tumor
  13. growth: a novel therapy for invasive bladder cancer. J. Urol. 1998,159, 282.
  14. [5] Borgstrom, P., Bourdon, M. A., Hillan, K. J., Sriramaro, R, Ferrara,
  15. N.: Neutralizing anti-vascular endothelial growth factor antibody completely in-
  16. hibits angiogenesis and growth of human prostate carcinoma microtumors in vivo.
  17. Prostate 1998, 35,1-10.
  18. [6] Bugajski, A.: System naczyniowy guza, wielkość i lokalizacja jako czynnik pro-
  19. gnostyczny raka nerki. Urol. Pol. 1994, 47, 12-22.
  20. [7] Campbell, S. C: Northwestern Univ. Med. Sch. Chicago II, USA. Adranceas
  21. in angiogenesis research: relevance to urological Oncology (review). J. Urol. 1997,
  22. 158, 1663-1674.
  23. [8] Folkmann, J.: Angiogenesis in cancer, vascular and rheumatoid and other dise-
  24. ase. Nature Med. 1995,1, 27-31.
  25. [9] Folkmann, J., Langer, R. et al.: Angiogenesis inhibition and tumor regres-
  26. sion caused by heparin or heparin fragment in the presence of cortisone. Science
  27. 1983, 221, 719-725.
  28. [10] Fujioka, T., Hasegawa., M., Ogiju, K., Matsushita, Y., Sato, M., Kubo, T.:
  29. Antitumor effects of angiogenesis inhibitor fumagillol (TNP-470) against murine
  30. renal cell carcinoma. Brit. J. Urol. 1994, 74, 416-421.
  31. [11] Gimbrone, M., Cotran, R., Leapman, S., Folkman, J.; Tumor growth and
  32. neovascularization: an eiperimental model using the rabbit cornea. J. Natl. Can-
  33. cer Inst. 1974, 5, 413-427.
  34. [12] Herbst, C, Kosmehl, H., Stiller, K. J., Bernardt, A., Eiselt, M., Schu-
  35. bert, J.: "Evaluation of microvessel density by Computerized image analysis in
  36. human renall cell carcinoma - correlation to pT category, nuclear grade, prolifera-
  37. tive actwity and occurrence of metastasis. J. Cancer Res. Clin. Oncol. 1998,124,
  38. 141-147.
  39. [13] Jones, A., Fujiyama, C: Angiogenesis in urological malignancy. Prognostic
  40. indicator and therapeutic target. B. J. Urology International 1999, 83, 535-556.
  41. [14] MacLenan, G. T., Bostick, D. G.: Microvessel density in renal cell carcino-
  42. ma. Lack of prognostic significance. Urology 1995, 46, 27-30.
  43. [15] Nativ, O:; Sabo, E., Reiss, A., Wald, M., Madjar, S., Moskovitz, B.:
  44. Clinical significance of tumor angiogenesis in patients with localized renal cell
  45. carcinoma. Urology 1998, 51, 693-696.
  46. [16] O'Reilly, M. S., Boehem, T., Shing, Y., Fukai, N., Vasios, G., Lane, W.
  47. S., Flynn, E., Birkhead, J. R., Olsen, B. R., Folkman, J.: Endostatin: an
  48. endogenous inhibitor of angiogenesis and tumor growth. Cell 1997, 88, 277-285.
  49. [17] O'Reilly, M. S., Holmgren, L., Chen, C. C, Folkman, J.: Angiostatin
  50. induces and sustains dormancy of human primary tumors in mince. Nature Med.
  51. 1996, 2, 689-692.
  52. [18] Skopińska-Różewska, E., Sommer, E., Pastewka, K., Barcz, E., Ka-
  53. zoń, M., Białas-Chromiec, B., Bałan, B., Przybyszewska, M., Janik,
  54. P.: Inhibitory effect of the obromine on angiogenic activity of human kidney and
  55. bladder cancer cells. Int. J. Molecular Med. 1998 (w druku).
  56. [19] Sommer, E., Skopińska-Różewska, E.: Anty'nowotworowe działanie nieste-
  57. rydowych leków przeciwzapalnych ze szczególnym uwzględnieniem sulindaku.
  58. Terapia 7, 1998.
  59. [20] Weidner, N.: Intratumoral vasculańty as a prognostic factor in cancers of the
  60. urogenital tract. Eur. J. Cancer 1996, 32A, 2506-2512.
  61. [21] Yoshino, S., Kato, M., Okada, K.: Pognostic significance of micmuessel co-
  62. unt in low stage renal cell carcinoma. Int. J. Urol. 1995, 2, 156-160.